[THE INTERPRETATION OF RESULTS OF DETECTION OF AGENTS OF VIRAL DIARRHEA IN REAL-TIME MODE].

ABSTRACT

The study was carried out to establish values of parameters characterizing concentrations of pathogens (threshold cycle - Ct) correlating with acute phase of viral gastroenteritis. The groups of patients with sporadic and group morbidity of acute intestinal infections were examined. The reagents kits Amplience (The central research institute of epidemiology, Russia), in real-time format polymerase chain reaction were applied to detect Rotavirus grA, Norovirus GII, Astrovirus, Adenovirus grF, Shigella spp, EIEC, Salmonella spp, Campylobacter spp (thermophilic group).The analysis was applied to distribution of Ct depending on isolated and combined detection ofpathogens in clinical samples. The evaluation was implemented concerning effect on Ct values of both inhibitors of polymerase chain reaction contained in feces and application of various amplifiers such as Rotor-Gene Q (QIAGEN, Germany), CFX96 (Bio-Rad, USA), "DT-96" (DNA technology, Russia). The risks of cross contamination during carrying out of investigations are evaluated. The asymmetric or bi-modal character of distribution of Ct values related to cases of combined detection of several pathogens is established. The following indicators are established common for patients with mono-infections (Ct mean ± SD) Rotavirus grA (Ct 20.63 ± 6.35; n = 978), Norovirus GII Astrovirus (Ct 21.06 ± 6.54; n = 54), Adenovirus grF (Ct 8.42 ± 2.4; n = 42). The corresponding values for victims of infective episodes amounted to Norovirus GII (24.19 ± 5.29; n = 447) and Rotavirus grA (18.65 ± 4.16; n = 50). The recommendations are presented concerning practical interpretation of results of real-time polymerase chain reaction. The indirect characteristic of content of pathogens in samples of clinical material derived from real-time polymerase chain reaction provides important information about association of pathogen with acute phase of disease. The high informativeness of given type of investigations support possibility of its effective implementation not only doing etiologic diagnostic but also in case of isolation of pathogen in clinically healthy individuals and examination of objects of environment.