High-Throughput Microarray Incubations Using Multi-Well Chambers.
Methods Mol Biol
; 1352: 19-26, 2016.
Article
em En
| MEDLINE
| ID: mdl-26490464
ABSTRACT
Peptide microarrays are ideal tools for a variety of applications ranging from epitope mapping to immune monitoring. Here we present a method for high-throughput screening of biological samples using only standard microtiter plate equipment. Parallel incubation of a large number of samples with a small library of peptides is enabled by printing multiple identical mini-arrays on one microarray slide and further combining four slides to yield an incubation frame possessing the dimensions of a 96-well microtiter plate. Applying conventional lab equipment such as ELISA washers, hundreds of samples can be processed in 1 day yielding approx. 200 data points in triplicates per sample.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Análise Serial de Proteínas
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article