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Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA.
Reddy, Teegala Lakshminarayan; Krishnarao, P Sivarama; Rao, Garikapati Koteswara; Bhimireddy, Eswar; Venkateswarlu, P; Mohapatra, Debendra K; Yadav, J S; Bhadra, Utpal; Bhadra, Manika Pal.
Afiliação
  • Reddy TL; Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.
  • Krishnarao PS; Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.
  • Rao GK; Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.
  • Bhimireddy E; Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.
  • Venkateswarlu P; Department of Chemistry, Sri Venkateswara University, Tirpupati, India.
  • Mohapatra DK; Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.
  • Yadav JS; Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.
  • Bhadra U; Functional Genomics and Gene Silencing Group, CSIR-Indian Institute of Chemical Technology, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
  • Bhadra MP; Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.
Int J Nanomedicine ; 10: 6411-23, 2015.
Article em En | MEDLINE | ID: mdl-26491299
ABSTRACT
A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Benzoatos / Materiais Biocompatíveis / Sistemas de Liberação de Medicamentos / Proteínas Proto-Oncogênicas c-bcl-2 / RNA Interferente Pequeno / Nanopartículas / Neoplasias Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Benzoatos / Materiais Biocompatíveis / Sistemas de Liberação de Medicamentos / Proteínas Proto-Oncogênicas c-bcl-2 / RNA Interferente Pequeno / Nanopartículas / Neoplasias Idioma: En Ano de publicação: 2015 Tipo de documento: Article