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Design of Enzyme-Encapsulated Protein Containers by In Vivo Crystal Engineering.
Abe, Satoshi; Ijiri, Hiroshi; Negishi, Hashiru; Yamanaka, Hiroyuki; Sasaki, Katsuhito; Hirata, Kunio; Mori, Hajime; Ueno, Takafumi.
Afiliação
  • Abe S; Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.
  • Ijiri H; Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.
  • Negishi H; Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.
  • Yamanaka H; Center for Advanced Insect Research Promotion, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
  • Sasaki K; Center for Advanced Insect Research Promotion, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
  • Hirata K; SR Life Science Instrumentation Unit, RIKEN/SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.
  • Mori H; Center for Advanced Insect Research Promotion, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
  • Ueno T; Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.
Adv Mater ; 27(48): 7951-6, 2015 Dec 22.
Article em En | MEDLINE | ID: mdl-26503073
ABSTRACT
Crystalline protein assemblies of polyhedra crystal (PhC) can be utilized as solid enzyme containers for long-term storage of enzymes with retention of their enzymatic activity. The enzymes can be released from the crystals at the optimum pH for the enzymatic activity by dissolution of the crystals using in vivo crystal engineering.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteína Quinase C / Engenharia de Proteínas / Proteínas Estruturais Virais Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteína Quinase C / Engenharia de Proteínas / Proteínas Estruturais Virais Idioma: En Ano de publicação: 2015 Tipo de documento: Article