DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil.
PLoS One
; 10(10): e0140636, 2015.
Article
em En
| MEDLINE
| ID: mdl-26506007
ABSTRACT
DNA barcoding has been an effective tool for species identification in several animal groups. Here, we used DNA barcoding to discriminate between 47 morphologically distinct species of Brazilian sand flies. DNA barcodes correctly identified approximately 90% of the sampled taxa (42 morphologically distinct species) using clustering based on neighbor-joining distance, of which four species showed comparatively higher maximum values of divergence (range 4.23-19.04%), indicating cryptic diversity. The DNA barcodes also corroborated the resurrection of two species within the shannoni complex and provided an efficient tool to differentiate between morphologically indistinguishable females of closely related species. Taken together, our results validate the effectiveness of DNA barcoding for species identification and the discovery of cryptic diversity in sand flies from Brazil.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Psychodidae
/
DNA
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Código de Barras de DNA Taxonômico
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article