Rational Substitution of Surface Acidic Residues for Enhancing the Thermostability of Thermolysin.

Zhu, Fucheng; Zhuang, Yu; Wu, Bin; Li, Jiahuang; He, Bingfang

Zhu, Fucheng; Zhuang, Yu; Wu, Bin; Li, Jiahuang; He, Bingfang.

Afiliação

Zhu F; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing, 211816, China.
Zhuang Y; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing, 211816, China.
Wu B; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing, 211816, China.
Li J; State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, 210093, China. lijiah@nju.edu.cn.
He B; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing, 211816, China. bingfanghe@njtech.edu.cn.

Appl Biochem Biotechnol ; 178(4): 725-38, 2016 Feb.

Article em En | MEDLINE | ID: mdl-26515979

Assuntos

Ácidos/química; Termolisina/metabolismo; Estabilidade Enzimática; Cinética; Simulação de Dinâmica Molecular; Mutagênese; Desdobramento de Proteína; Temperatura; Termolisina/genética

Palavras-chave

Molecular dynamic simulation; Surface acidic residue; Thermolysin; Thermostability

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos / Termolisina Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos / Termolisina Idioma: En Ano de publicação: 2016 Tipo de documento: Article