Structural characterization and anti-tumor activity of polysaccharide produced by Hirsutella sinensis.
Int J Biol Macromol
; 82: 959-66, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26517958
ABSTRACT
HSP-III, a novel homogeneous polysaccharide with 513.89 kDa molecular weight, was fractionated from submerged cultures of Hirsutella sinensis by Sevag and chromatography on Sephadex G-100 column. The total sugar content of HSP-III was amounted to 89.87%. Based on the results of high performance gel permeation chromatogram (HPGPC), FT-IR, NMR spectroscopy, GC, periodate oxidation-smith degradation and methylation analysis, it showed that HSP-III was mainly composed of mannose and galactose, and a small amount of rhamnose, arabinose, xylose, and glucose. The molar ratio of RhaAraXylManGluGal was 1.002.4413.1174.1313.8054.39. The main chain of HSP-III was majorly composed of (1â3) glucose. The tumor inhibition ratio on H22 cell was 79.04% at 100 µg/mL of HSP-III.
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MEDLINE
Assunto principal:
Ascomicetos
/
Polissacarídeos Fúngicos
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Antineoplásicos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article