Structure of Escherichia coli tryptophanase purified from an alkaline-stressed bacterial culture.
Acta Crystallogr F Struct Biol Commun
; 71(Pt 11): 1378-83, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26527264
ABSTRACT
Tryptophanase is a bacterial enzyme involved in the degradation of tryptophan to indole, pyruvate and ammonia, which are compounds that are essential for bacterial survival. Tryptophanase is often overexpressed in stressed cultures. Large amounts of endogenous tryptophanase were purified from Escherichia coli BL21 strain overexpressing another recombinant protein. Tryptophanase was crystallized in space group P6522 in the apo form without pyridoxal 5'-phosphate bound in the active site.
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Base de dados:
MEDLINE
Assunto principal:
Triptofanase
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Proteínas de Escherichia coli
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Escherichia coli
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Antiácidos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article