[Improvement of natamycin production in an industrial strain by heterologous expression of the afsRS(cla) global regulatory genes].
Sheng Wu Gong Cheng Xue Bao
; 31(5): 744-51, 2015 May.
Article
em Zh
| MEDLINE
| ID: mdl-26571695
ABSTRACT
The afsRS(cla) global regulatory genes from Streptomyces clavuligerus activate the production of two antibiotics in Streptomyces lividans. In this study, we gained an increase of 38% in the production of natamycin (3.56 g/L) in an industrial strain Streptomyces gilvosporeus TZ1401 through the integration of pHL851 that bears the afsRS(cla) global regulatory genes into its genome. We discovered by quantitive real-time reverse transcription PCR (qRT-PCR) that the expression of 6 genes of the natamycin biosynthetic gene cluster were improved from 1.9 to 2.7 times. This suggests that afsRS(cla) improve the production of natamycin through increased transcription. This study provides a good example for applying afsRS(cla) in high yield breeding of industrial antibiotic producers.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Microbiologia Industrial
/
Genes Reguladores
/
Natamicina
Idioma:
Zh
Ano de publicação:
2015
Tipo de documento:
Article