Your browser doesn't support javascript.
loading
Protein labeling in Escherichia coli with (2)H, (13)C, and (15)N.
Hoopes, J Todd; Elberson, Margaret A; Preston, Renae J; Reddy, Prasad T; Kelman, Zvi.
Afiliação
  • Hoopes JT; Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA. Electronic address: hoopesj@umd.edu.
  • Elberson MA; Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.
  • Preston RJ; Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.
  • Reddy PT; Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA; Biomolecular Labeling Laboratory, and Biomolecular Structure and Function Group, Institute for Bioscience an
  • Kelman Z; Biomolecular Labeling Laboratory, and Biomolecular Structure and Function Group, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.
Methods Enzymol ; 565: 27-44, 2015.
Article em En | MEDLINE | ID: mdl-26577726
A number of structural biology techniques such as nuclear magnetic resonance spectroscopy and small-angle neutron scattering can be performed with proteins with nuclei at natural isotope abundance. However, the use of proteins labeled with stable isotopes ((2)H, (13)C, and (15)N) enables greater experimental flexibility. In this chapter, several methods for uniform and fractional protein labeling with stable isotopes using Escherichia coli in a defined media are described. The methods described can be used for labeling with single or multiple isotopes.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Escherichia coli / Escherichia coli / Marcação por Isótopo Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Escherichia coli / Escherichia coli / Marcação por Isótopo Idioma: En Ano de publicação: 2015 Tipo de documento: Article