Transient Protein Expression by Agroinfiltration in Lettuce.
Methods Mol Biol
; 1385: 55-67, 2016.
Article
em En
| MEDLINE
| ID: mdl-26614281
ABSTRACT
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.
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Base de dados:
MEDLINE
Assunto principal:
Transformação Genética
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Proteínas Recombinantes
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Engenharia Genética
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Agrobacterium tumefaciens
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Lactuca
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Vetores Genéticos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article