Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures.
Cell Rep
; 13(9): 1765-71, 2015 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-26628361
ABSTRACT
Protamines confer a compact structure to the genome of male gametes. Here, we find that somatic cells can be remodeled by transient expression of protamine 1 (Prm1). Ectopically expressed Prm1 forms scattered foci in the nuclei of fibroblasts, which coalescence into spermatid-like structures, concomitant with a loss of histones and a reprogramming barrier, H3 lysine 9 methylation. Protaminized nuclei injected into enucleated oocytes efficiently underwent protamine to maternal histone TH2B exchange and developed into normal blastocyst stage embryos in vitro. Altogether, our findings present a model to study male-specific chromatin remodeling, which can be exploited for the improvement of somatic cell nuclear transfer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Protaminas
/
Núcleo Celular
/
Montagem e Desmontagem da Cromatina
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article