Dihydromyricetin induces cell apoptosis via a p53-related pathway in AGS human gastric cancer cells.
Genet Mol Res
; 14(4): 15564-71, 2015 Dec 02.
Article
em En
| MEDLINE
| ID: mdl-26634523
ABSTRACT
The aim of the present study was to determine the anti-proliferative and pro-apoptotic effects of dihydromyricetin (DHM) on the AGS human gastric cancer cells and their underlying mechanisms. The effects of DHM on AGS cells were evaluated by using 3-(4, 5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase, and Annexin V/propidium iodide (PI) double-staining assays. The underlying mechanisms were determined by using quantitative real-time polymerase chain reaction. The results demonstrated that DHM significantly (P < 0.05) inhibited AGS cell proliferation and induced cell cytotoxicity in a dose- and time-dependent manner. Additionally, Annexin V/PI double-staining assay showed that DHM promoted cell apoptosis in both, early and late stages. Furthermore, DHM also regulated the expression of apoptotic genes such as p53 and B-cell lymphoma-2 (bcl-2) in a dose- and time-dependent manner. In conclusion, this is the first report demonstrating the anticancer and pro-apoptosis effects of DHM on AGS human gastric cancer cells. The results strongly suggest that DHM may be a potential therapeutic candidate for the treatment of gastric cancer.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
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Transdução de Sinais
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Proteína Supressora de Tumor p53
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Apoptose
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Flavonóis
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Antineoplásicos Fitogênicos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article