Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation.
Elife
; 42015 Dec 08.
Article
em En
| MEDLINE
| ID: mdl-26646180
ABSTRACT
The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively and remotely controls Ca(2+) oscillations and Ca(2+)-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca(2+)-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded 'photoactivatable adjuvant' to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote and wireless control of Ca(2+)-modulated activities with tailored function.
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Base de dados:
MEDLINE
Assunto principal:
Sinalização do Cálcio
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Imunomodulação
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Optogenética
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Raios Infravermelhos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article