B'-protein phosphatase 2A is a functional binding partner of delta-retroviral integrase.
Nucleic Acids Res
; 44(1): 364-76, 2016 Jan 08.
Article
em En
| MEDLINE
| ID: mdl-26657642
ABSTRACT
To establish infection, a retrovirus must insert a DNA copy of its RNA genome into host chromatin. This reaction is catalysed by the virally encoded enzyme integrase (IN) and is facilitated by viral genus-specific host factors. Herein, cellular serine/threonine protein phosphatase 2A (PP2A) is identified as a functional IN binding partner exclusive to δ-retroviruses, including human T cell lymphotropic virus type 1 and 2 (HTLV-1 and HTLV-2) and bovine leukaemia virus (BLV). PP2A is a heterotrimer composed of a scaffold, catalytic and one of any of four families of regulatory subunits, and the interaction is specific to the B' family of the regulatory subunits. B'-PP2A and HTLV-1 IN display nuclear co-localization, and the B' subunit stimulates concerted strand transfer activity of δ-retroviral INs in vitro. The protein-protein interaction interface maps to a patch of highly conserved residues on B', which when mutated render B' incapable of binding to and stimulating HTLV-1 and -2 IN strand transfer activity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Deltaretrovirus
/
Integrases
/
Proteína Fosfatase 2
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article