Evaluation of phenotypic and molecular methods for identification of Streptococcus pneumoniae.
Future Microbiol
; 11(1): 43-50, 2016.
Article
em En
| MEDLINE
| ID: mdl-26673889
ABSTRACT
AIM:
The objective of this study is to compare various Streptococcus pneumoniae identification methods. MATERIALS &METHODS:
In total, 1371 putative S. pneumoniae isolates were tested with three phenotypic methods and a molecular-based method targeting a virulence factor (CpsA). We assessed the sensitivity and the specificity of each method and widely used S. pneumoniae identification algorithm.RESULTS:
None of the methods or the identification algorithm used separately was able to correctly identify all S. pneumoniae isolates. Furthermore, a high rate of optochin resistance was found.CONCLUSIONS:
We demonstrated the failure of the current S. pneumoniae identification methods and optochin susceptibility-based algorithm. In addition, the high rate of optochin resistance might justify the necessity of a close monitoring of optochin susceptibility.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Streptococcus pneumoniae
/
Técnicas Bacteriológicas
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Técnicas de Diagnóstico Molecular
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article