Clinical events and their relation to the tumor necrosis factor-alpha and interleukin-10 genotypes in Sickle-Cell-Anemia patients.
Hematol Oncol Stem Cell Ther
; 9(1): 14-9, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26686970
ABSTRACT
OBJECTIVE/BACKGROUND:
Sickle-cell anemia (SCA) is a genetic blood disease characterized by chronic inflammation and a heterogeneous clinical picture. Serum tumor necrosis factor (TNF-alpha) and interleukin 10 (IL-10) levels are associated with the clinical course of SCA. This study aimed to evaluate the association between the frequency of the polymorphisms TNF-alpha-308 GâA, IL-10-1082 GâA, IL-10-819 CâT, and IL-10-592 AâC; serum TNF-alpha; and IL-10 levels, and the incidence of clinical events in SCA patients.METHODS:
Polymerase chain reaction-restriction fragment length polymorphism and enzyme-linked immunosorbent assay were performed on 25 adults with SCA at the steady state; their data were compared with those for 26 healthy individuals.RESULTS:
The most frequent genotype of the TNF-alpha polymorphism was GG (low producer), and the most frequent genotype of the IL-10 polymorphisms was "low producer" (ACC ACC, ACC ATA, ATA ATA). The TNF-alpha levels were significantly higher in SCA in patients with acute chest syndrome (ACS). The IL-10 levels were reduced in polytransfusion and in patients with ACS.CONCLUSION:
The patients presented prevalence of TNF-alpha and IL-10 low-profile producer. The cytokine serum levels presented an association with the presence of polytransfusion and ACS in SCA patients.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Fator de Necrose Tumoral alfa
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Interleucina-10
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Polimorfismo de Nucleotídeo Único
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Anemia Falciforme
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article