Purification of Na,K-ATPase from Pig Kidney.
Methods Mol Biol
; 1377: 5-10, 2016.
Article
em En
| MEDLINE
| ID: mdl-26695017
ABSTRACT
The method of purification of Na,K-ATPase from pig kidney is based on a differential centrifugation and SDS-treatment of a microsomal preparation. The yield is 0.4 mg protein per 1 g tissue with the specific (ouabain-sensitive) activity of 25-28 µmol Pi/min per mg protein and nucleotide binding capacity of 3 nmol/mg. The protein/lipid ratio is 1/1 (mg/mg) with a protein purity of ~80 %.
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Base de dados:
MEDLINE
Assunto principal:
ATPase Trocadora de Sódio-Potássio
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Rim
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Biologia Molecular
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article