Expression of Na,K-ATPase and H,K-ATPase Isoforms with the Baculovirus Expression System.
Methods Mol Biol
; 1377: 71-8, 2016.
Article
em En
| MEDLINE
| ID: mdl-26695023
ABSTRACT
P-type ATPases can be expressed in several cell systems. The baculovirus expressions system uses an insect virus to enter and express proteins in Sf9 insect cells. This expression system is a lytic system in which the cells will die a few days after viral infection. Subsequently, the expressed proteins can be isolated. Insect cells are a perfect system to study P-type ATPases as they have little or no endogenous Na,K-ATPase activity and other ATPase activities can be inhibited easily. Here we describe in detail the expression and isolation of Na,K-ATPase and H,K-ATPase isoforms with the baculovirus expression system.
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MEDLINE
Assunto principal:
ATPase Trocadora de Sódio-Potássio
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ATPase Trocadora de Hidrogênio-Potássio
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Isoformas de Proteínas
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Biologia Molecular
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article