Usefulness of a novel multiplex real-time PCR assay for the diagnosis of sexually-transmitted infections.
Enferm Infecc Microbiol Clin
; 34(8): 471-6, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-26706392
ABSTRACT
INTRODUCTION:
Sexually transmitted infections (STI) are currently on the increase worldwide. New molecular tools have been developed in the past few years in order to improve their diagnosis. An evaluation was carried out using a new commercially available real-time PCR assay, Anyplex™ II STI-7 (Seegene, Seoul, Korea), which detects seven major pathogens in a single reaction - Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, and Ureaplasma parvum - and compared with conventional methods performed in our laboratory. MATERIALS ANDMETHODS:
Two different populations were included, and 267 specimens from different sites of infection (urines, endocervical swabs, rectal swabs, vaginal swabs, urethral swabs and one inguinal adenopathy) were processed for both methods.RESULTS:
The parameters of clinical performance were calculated for C. trachomatis, N. gonorrhoeae, and T. vaginalis, and the assay achieved sensitivities (SE) from 93.94% to 100%, and specificities (SP) from 96.55% to 100%, with negative predictive values (NPV) from 93.33% to 98.85%, and positive predictive values (PPV) from 96.88% to 100%, with a very good agreement (kappa index from 0.88 to 1).CONCLUSIONS:
Anyplex™ II STI-7 is a good tool for the reliable diagnosis of STI. Its ease of use and processing allows it to be incorporated into the day to day laboratory work.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Infecções Sexualmente Transmissíveis
/
Reação em Cadeia da Polimerase Multiplex
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article