CED-4 is an mRNA-binding protein that delivers ced-3 mRNA to ribosomes.
Biochem Biophys Res Commun
; 470(1): 48-53, 2016 Jan 29.
Article
em En
| MEDLINE
| ID: mdl-26740177
ABSTRACT
Cell death abnormal (ced)-3 and ced-4 genes regulate apoptosis to maintain tissue homeostasis in Caenorhabditis elegans. Apoptosome formation and CED-4 translocation drive CED-3 activation. However, the precise role of CED-4 translocation is not yet fully understood. In this study, using a combination of immunoprecipitation and reverse transcription-polymerase chain reaction methods in cells and a glutathione-S-transferase pull down assay in a cell-free system, we show that CED-4 binds ced-3 mRNA. In the presence of ced-3 mRNA, CED-4 protein is enriched in the microsomal fraction and interacts with ribosomal protein L10a in mammalian cells, increasing the levels of CED-3. These results suggest that CED-4 forms a complex with ced-3 mRNA and delivers it to ribosomes for translation.
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MEDLINE
Assunto principal:
Ribossomos
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Proteínas de Ligação ao Cálcio
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Caspases
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Proteínas de Caenorhabditis elegans
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MicroRNAs
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article