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Mildly acidic conditions eliminate deamidation artifact during proteolysis: digestion with endoprotease Glu-C at pH 4.5.
Liu, Shanshan; Moulton, Kevin Ryan; Auclair, Jared Robert; Zhou, Zhaohui Sunny.
Afiliação
  • Liu S; Department of Chemistry and Chemical Biology, Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, 02115, USA.
  • Moulton KR; Department of Chemistry and Chemical Biology, Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, 02115, USA.
  • Auclair JR; Department of Chemistry and Chemical Biology, Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, 02115, USA.
  • Zhou ZS; Department of Chemistry and Chemical Biology, Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, 02115, USA. z.zhou@neu.edu.
Amino Acids ; 48(4): 1059-1067, 2016 Apr.
Article em En | MEDLINE | ID: mdl-26748652
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Asparagina / Serina Endopeptidases / Artefatos / Ácido Aspártico / Ácido Isoaspártico / Amidas Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Asparagina / Serina Endopeptidases / Artefatos / Ácido Aspártico / Ácido Isoaspártico / Amidas Idioma: En Ano de publicação: 2016 Tipo de documento: Article