[Affine Haemosorbents Based on Aromatic Peptides for Binding of the Immunoglobulin G].

ABSTRACT

Affinity haemoadsorbents based on WY, WTY, WNY ligands and polysaccharide matrix were developed for the human immunoglobulin G binding. The characteristics of new sorbents such as the binding of total IgG and binding of IgG subclasses were compared. It was found that all new sorbents well extract the IgG from the blood plasma. It was evidenced that WNY-based sorbent is more effective for binding of IgG subclass 3. The determination of physic-chemical characteristics of IgG binding revealed that desorption constants for IgG are 10 ± 3, 28 ± 4 and 13 ± 3 µM for WY, WTY, WNY based sorbents respectively. Maximum sorption capacities for IgG are 43 ± 2, 45 ± 3 and 46 ± 3 mg IgG per ml of sorbent for WY, WTY, WNY based sorbents respectively. Also it was shown that the new sorbents are compatible with blood and are suitable for the medical purposes.