Engineering of bacterial exotoxins for highly efficient and receptor-specific intracellular delivery of diverse cargos.
Biotechnol Bioeng
; 113(8): 1639-46, 2016 08.
Article
em En
| MEDLINE
| ID: mdl-26773973
ABSTRACT
The intracellular delivery of proteins with high efficiency in a receptor-specific manner is of great significance in molecular medicine and biotechnology, but remains a challenge. Herein, we present the development of a highly efficient and receptor-specific delivery platform for protein cargos by combining the receptor binding domain of Escherichia coli Shiga-like toxin and the translocation domain of Pseudomonas aeruginosa exotoxin A. We demonstrated the utility and efficiency of the delivery platform by showing a cytosolic delivery of diverse proteins both in vitro and in vivo in a receptor-specific manner. In particular, the delivery system was shown to be effective for targeting an intracellular protein and consequently suppressing the tumor growth in xenograft mice. The present platform can be widely used for intracellular delivery of diverse functional macromolecules with high efficiency in a receptor-specific manner. Biotechnol. Bioeng. 2016;113 1639-1646. © 2016 Wiley Periodicals, Inc.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Proteínas Recombinantes de Fusão
/
Engenharia de Proteínas
/
Sistemas de Liberação de Medicamentos
/
ADP Ribose Transferases
/
Toxinas Shiga
/
Fatores de Virulência
/
Espaço Intracelular
/
Exotoxinas
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article