Cyclodextrin-assisted assembly of PEGylated polyester nanoparticles decorated with folate.
Colloids Surf B Biointerfaces
; 141: 148-157, 2016 May 01.
Article
em En
| MEDLINE
| ID: mdl-26852098
ABSTRACT
In the last decades, nano-oncologicals bearing a polyethylene glycol (PEG) coating are being emerging as biomimetic devices able to drive their drug cargo to solid tumors through passive mechanisms. To improve selectivity toward cancer cells, nanocarriers decorated with the small ligand folate have been widely investigated. Nevertheless, a great challenge remains the effective exposition of folate on nanoparticles (NPs), which is a key prerequisite to ensure the correct binding to receptor and the following endocytic uptake. On these premises, in this study we propose a novel strategy to produce core-shell folate-targeted NPs based on diblock copolymers of poly(ε-caprolactone) (PCL) and PEG through the aid of (2-hydroxypropyl)-ß-cyclodextrin (HPßCD). PCL4300-PEG2000 and PCL4300-PEG2000-Fol copolymers were synthesized, characterized and employed to produce NPs without and with HPßCD by a melting/sonication procedure. Colloidal properties of targeted NPs produced with HPßCD demonstrated a highly extended conformation of PEG chains in the shell, an enhanced interaction with a specific antibody against folate and a higher uptake in cells overexpressing folate receptor. Overall, these results suggest that proper manipulation of PEG shell conformation through HPßCD can represent a novel non-covalent strategy to modify shell features.
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Base de dados:
MEDLINE
Assunto principal:
Poliésteres
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Polietilenoglicóis
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Beta-Ciclodextrinas
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Nanopartículas
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Ácido Fólico
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article