T cell-specific inactivation of mouse CD2 by CRISPR/Cas9.
Sci Rep
; 6: 21377, 2016 Feb 23.
Article
em En
| MEDLINE
| ID: mdl-26903281
ABSTRACT
The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4(+) and CD8(+) lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells lacking CD2 carryied mutations, which confirmed that Cas9 driven by cell-type specific promoters can edit genes in the mouse and may thus allow targeted studies of gene function in vivo.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Guia de Cinetoplastídeos
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Antígenos CD2
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Inativação Gênica
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Sistemas CRISPR-Cas
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Edição de Genes
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article