LC-coupled ESI MS for quantification of miltefosine in human and hamster plasma.
Bioanalysis
; 8(6): 533-45, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26915470
ABSTRACT
BACKGROUND:
Leishmaniasis, a fatal parasitic disease, is the second largest parasitic killer in the world and miltefosine is the first and only oral drug available for its treatment.RESULTS:
A rapid, sensitive and simple LC-MS/MS method for miltefosine quantification in hamster and human plasma was developed and validated over the range of 2.5-400 ng/ml. The mass spectrometric detection of the drug was carried out in multiple reaction monitoring mode (m/z 408.1â125.1) using an electrospray positive ionization. The protein precipitation method was employed for sample (50 µl) cleanup.CONCLUSION:
The proposed method provided accurate and precise high-throughput quantification of miltefosine in plasma and was successfully applied to its oral PK study in Golden Syrian hamsters.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosforilcolina
/
Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas por Ionização por Electrospray
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Antiprotozoários
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article