Labeling and Selective Inactivation of Gram-Positive Bacteria Employing Bimodal Photoprobes with Dual Readouts.
Chemistry
; 22(15): 5243-52, 2016 Apr 04.
Article
em En
| MEDLINE
| ID: mdl-26929124
ABSTRACT
Carbohydrate-conjugated silicon(IV) phthalocyanines with bimodal photoactivity were developed as probes with both fluorescent labeling and photosensitizing capabilities, and the concomitant fluorescent labeling and photoinduced inactivation of Gram-positive and Gram-negative models was explored. The maltohexaose-conjugated photoprobe provides a dual readout to distinguish between both groups of pathogens, as only the Gram-positive species was inactivated, even though both appeared labeled with near-infrared luminescence. Antibiotic resistance did not hinder the phototoxic effect, as even the methicillin-resistant pathogen Staphylococcus aureus (MRSA) was completely photoinactivated. Time-resolved confocal fluorescence microscopy analysis suggests that the photoprobe sticks onto the outer rim of the microorganisms, explaining the resistance of Gram-negative species on the basis of their membrane constitution. The mannose-conjugated photoprobe yields a different readout because it is able to label and to inactivate only the Gram-positive strain.
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Base de dados:
MEDLINE
Assunto principal:
Silanos
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Staphylococcus aureus
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Carboidratos
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Fármacos Fotossensibilizantes
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Staphylococcus aureus Resistente à Meticilina
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Bactérias Gram-Positivas
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Indóis
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article