Dietary saturated fatty acid and polyunsaturated fatty acid oppositely affect hepatic NOD-like receptor protein 3 inflammasome through regulating nuclear factor-kappa B activation.
World J Gastroenterol
; 22(8): 2533-44, 2016 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-26937141
ABSTRACT
AIM:
To investigate the effect of different dietary fatty acids on hepatic inflammasome activation.METHODS:
Wild-type C57BL/6 mice were fed either a high-fat diet or polyunsaturated fatty acid (PUFA)-enriched diet. Primary hepatocytes were treated with either saturated fatty acids (SFAs) or PUFAs as well as combined with lipopolysaccharide (LPS). The expression of NOD-like receptor protein 3 (NLRP3) inflammasome, peroxisome proliferator-activated receptor-γ and nuclear factor-kappa B (NF-κB) was determined by real-time PCR and Western blot. The activity of Caspase-1 and interleukine-1ß production were measured.RESULTS:
High-fat diet-induced hepatic steatosis was sufficient to induce and activate hepatic NLRP3 inflammasome. SFA palmitic acid (PA) directly activated NLRP3 inflammasome and increased sensitization to LPS-induced inflammasome activation in hepatocytes. In contrast, PUFA docosahexaenoic acid (DHA) had the potential to inhibit NLRP3 inflammasome expression in hepatocytes and partly abolished LPS-induced NLRP3 inflammasome activation. Furthermore, a high-fat diet increased but PUFA-enriched diet decreased sensitization to LPS-induced hepatic NLRP3 inflammasome activation in vivo. Moreover, PA increased but DHA decreased phosphorylated NF-κB p65 protein expression in hepatocytes.CONCLUSION:
Hepatic NLRP3 inflammasome activation played an important role in the development of non-alcoholic fatty liver disease. Dietary SFAs and PUFAs oppositely regulated the activity of NLRP3 inflammasome through direct activation or inhibition of NF-κB.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Docosa-Hexaenoicos
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Ácido Palmítico
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Hepatócitos
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Fator de Transcrição RelA
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Inflamassomos
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Hepatopatia Gordurosa não Alcoólica
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Proteína 3 que Contém Domínio de Pirina da Família NLR
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Fígado
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article