The localization of the divergent beta 2-tubulin isotype in the microtubular arrays of Physarum polycephalum.
J Cell Sci
; 94 ( Pt 2): 217-26, 1989 Oct.
Article
em En
| MEDLINE
| ID: mdl-2695530
ABSTRACT
The beta 2-tubulin isotype of Physarum polycephalum is only 83% identical in amino acid sequence with the constitutively expressed beta 1B-tubulin and the myxamoeba-specific beta 1A-tubulin isotypes. A polyclonal antibody specific for beta 2-tubulin was used to monitor the subcellular distribution of the beta 2-tubulin antigen in the mitotic spindle of the mature plasmodium - the sole microtubular array in that stage of Physarum. By immunofluorescence, the beta 2-tubulin antigen was detected throughout this anastral mitotic spindle, at all stages of mitosis. Physarum myxamoebae contain astral mitotic spindles and cytoskeletal microtubules. No beta 2-tubulin antigen was detected in the myxamoebal stage. However, as cultures of myxamoebae developed into plasmodia, the beta 2-tubulin antigen was found in the astral mitotic spindles and cytoskeletons in developing cells. Thus, the presence of the plasmodial beta 2-tubulin isotype in a mitotic spindle does not determine a closed, anastral mitosis.
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Base de dados:
MEDLINE
Assunto principal:
Physarum
/
Tubulina (Proteína)
/
Microtúbulos
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article