Protein extracts from cultured cells contain nonspecific serum albumin.
Biosci Biotechnol Biochem
; 80(6): 1164-7, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-26967711
ABSTRACT
Serum is an important component of cell culture media. The present study demonstrates contamination of intracellular protein extract by bovine serum albumin from the culture media and illustrates how this contamination can cause the misinterpretation of western blot results. Preliminary experiments can prevent the misinterpretation of some experimental results, and optimization of the washing process may enable specific protein detection.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Soroalbumina Bovina
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Proteínas Sanguíneas
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Artefatos
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Extração Líquido-Líquido
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article