The Knotted Protein UCH-L1 Exhibits Partially Unfolded Forms under Native Conditions that Share Common Structural Features with Its Kinetic Folding Intermediates.

Lou, Shih-Chi; Wetzel, Svava; Zhang, Hongyu; Crone, Elizabeth W; Lee, Yun-Tzai; Jackson, Sophie E; Hsu, Shang-Te Danny

Lou, Shih-Chi; Wetzel, Svava; Zhang, Hongyu; Crone, Elizabeth W; Lee, Yun-Tzai; Jackson, Sophie E; Hsu, Shang-Te Danny.

Afiliação

Lou SC; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK; Institute of Biological Chemistry, Academia Sinica, 128, Section 2, Academia Road, Taipei 11529, Taiwan.
Wetzel S; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
Zhang H; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
Crone EW; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
Lee YT; Institute of Biological Chemistry, Academia Sinica, 128, Section 2, Academia Road, Taipei 11529, Taiwan; Institute of Biochemical Sciences, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
Jackson SE; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK. Electronic address: sej13@cam.ac.uk.
Hsu SD; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK; Institute of Biological Chemistry, Academia Sinica, 128, Section 2, Academia Road, Taipei 11529, Taiwan; Institute of Biochemical Sciences, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 106, T

J Mol Biol ; 428(11): 2507-2520, 2016 06 05.

Article em En | MEDLINE | ID: mdl-27067109

Assuntos

Ubiquitina Tiolesterase/metabolismo; Deutério/metabolismo; Medição da Troca de Deutério/métodos; Humanos; Hidrogênio/metabolismo; Concentração de Íons de Hidrogênio; Cinética; Espectroscopia de Ressonância Magnética/métodos; Conformação Proteica em alfa-Hélice/fisiologia; Conformação Proteica em Folha beta/fisiologia; Desnaturação Proteica; Dobramento de Proteína

Palavras-chave

folding intermediate; folding pathway; knotted protein; partially unfolded form; ubiquitin C-terminal hydrolase

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ubiquitina Tiolesterase Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ubiquitina Tiolesterase Idioma: En Ano de publicação: 2016 Tipo de documento: Article