Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation.
Dev Cell
; 37(2): 148-61, 2016 Apr 18.
Article
em En
| MEDLINE
| ID: mdl-27093084
ABSTRACT
During neurulation of chordate ascidians, the 11th mitotic division within the epidermal layer shows a posterior-to-anterior wave that is precisely coordinated with the unidirectional progression of the morphogenetic movement. Here we show that the first sign of this patterned mitosis is an asynchronous anterior-to-posterior S-phase length and that mitotic synchrony is reestablished by a compensatory asynchronous G2-phase length. Live imaging combined with genetic experiments demonstrated that compensatory G2-phase regulation requires transcriptional activation of the G2/M regulator cdc25 by the patterning genes GATA and AP-2. The downregulation of GATA and AP-2 at the onset of neurulation leads to loss of compensatory G2-phase regulation and promotes the transition to patterned mitosis. We propose that such developmentally regulated cell-cycle compensation provides an abrupt switch to spatially patterned mitosis in order to achieve the coordination between mitotic timing and morphogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ciclo Celular
/
Fosfatases cdc25
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Neurulação
/
Mitose
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Morfogênese
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article