Optical and nuclear imaging of glioblastoma with phosphatidylserine-targeted nanovesicles.
Oncotarget
; 7(22): 32866-75, 2016 May 31.
Article
em En
| MEDLINE
| ID: mdl-27096954
ABSTRACT
Multimodal tumor imaging with targeted nanoparticles potentially offers both enhanced specificity and sensitivity, leading to more precise cancer diagnosis and monitoring. We describe the synthesis and characterization of phenol-substituted, lipophilic orange and far-red fluorescent dyes and a simple radioiodination procedure to generate a dual (optical and nuclear) imaging probe. MALDI-ToF analyses revealed high iodination efficiency of the lipophilic reporters, achieved by electrophilic aromatic substitution using the chloramide 1,3,4,6-tetrachloro-3α,6α-diphenyl glycoluril (Iodogen) as the oxidizing agent in an organic/aqueous co-solvent mixture. Upon conjugation of iodine-127 or iodine-124-labeled reporters to tumor-targeting SapC-DOPS nanovesicles, optical (fluorescent) and PET imaging was performed in mice bearing intracranial glioblastomas. In addition, tumor vs non-tumor (normal brain) uptake was compared using iodine-125. These data provide proof-of-principle for the potential value of SapC-DOPS for multimodal imaging of glioblastoma, the most aggressive primary brain tumor.
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Base de dados:
MEDLINE
Assunto principal:
Fosfatidilserinas
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Neoplasias Encefálicas
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Glioblastoma
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Compostos Radiofarmacêuticos
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Saposinas
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Tomografia por Emissão de Pósitrons
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Imagem Multimodal
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Imagem Óptica
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Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article