Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals.
Mol Med Rep
; 13(6): 5077-83, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-27109489
ABSTRACT
Synucleinopathies and abnormalities in the nerves of the enteric nervous system are hypothesized to be involved in age-associated motility disorders. The aim of the present study was to investigate the expression of various antigens, including αsynuclein (Syn) and its posttranslational modified forms, in the human colon at various ages. In addition, the study aimed to correlate the expression of Syn with neurodegeneration. Immunohistochemistry was used to detect the expression of neurofilament (NF), Syn, as well as its nitrated (N) form in the healthy colonic tissue of 12 young (34.08±5.12 years), 10 middleaged (51.80±3.52 years), and 11 elderly (75.82±7.70 years) individuals. To the best of our knowledge, the current study is the first to demonstrate the presence of NSyn in the colonic tissue. NSyn was identified in the upper layer of the mucosa and submucosa layer. Furthermore, Syn (wildtype) was present in the mucosa and submucosa. The number of NFpositive neurons in the submucosal layer declined significantly with age (P<0.01). In addition, Syn and NSyn significantly increased during aging (P<0.01). Furthermore, a negative correlation was identified between neuron number and synucleinopathies, indicating the abnormal accumulation of both wild-type Syn and NSyn in the mucosa, submucosa, muscle layer and myenteric plexus. The present study demonstrates that the Syn pathology may be linked to colic neuronal degeneration during normal aging, and this link may cause functional deficits.
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Base de dados:
MEDLINE
Assunto principal:
Processamento de Proteína Pós-Traducional
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Colo
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Alfa-Sinucleína
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Degeneração Neural
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article