irCLIP platform for efficient characterization of protein-RNA interactions.
Nat Methods
; 13(6): 489-92, 2016 06.
Article
em En
| MEDLINE
| ID: mdl-27111506
ABSTRACT
The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
/
Proteínas de Ligação a RNA
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Imunoprecipitação
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article