Lysosomal ATP imaging in living cells by a water-soluble cationic polythiophene derivative.
Biosens Bioelectron
; 83: 213-20, 2016 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-27131993
ABSTRACT
Lysosomes in astrocytes and microglia can release ATP as the signaling molecule for the cells through ca(2+)-dependent exocytosis in response to various stimuli. At present, fluorescent probes that can detect ATP in lysosomes have not been reported. In this work, we have developed a new water-soluble cationic polythiophene derivative that can be specifically localized in lysosomes and can be utilized as a fluorescent probe to sense ATP in cells. PEMTEI exhibits high selectivity and sensitivity to ATP at physiological pH values and the detection limit of ATP is as low as 10(-11)M. The probe has low cytotoxicity, good permeability and high photostability in living cells and has been applied successfully to real-time monitoring of the change in concentrations of ATP in lysosomes though fluorescence microscopy. We also demonstrated that lysosomes in Hela cells can release ATP through Ca(2+)-dependent exocytosis in response to drug stimuli.
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MEDLINE
Assunto principal:
Polímeros
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Tiofenos
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Trifosfato de Adenosina
/
Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article