Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0.
Methods
; 105: 26-33, 2016 08 01.
Article
em En
| MEDLINE
| ID: mdl-27163865
ABSTRACT
AFS is a recently introduced high-throughput single-molecule technique that allows studying structural and mechanochemical properties of many biomolecules in parallel. To further improve the method, we developed a modelling tool to optimize the layer thicknesses, and a calibration method to experimentally validate the modelled force profiles. After optimization, we are able to apply 350pN on 4.5µm polystyrene beads, without the use of an amplifier, at the coverslip side of the AFS chip. Furthermore, we present the use of a transparent piezo to generate the acoustic force and we show that AFS can be combined with high-NA oil or water-immersion objectives. With this set of developments AFS will be applicable to a broad range of single-molecule experiments.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acústica
/
Ácidos Nucleicos
/
Microscopia de Força Atômica
/
Imagem Individual de Molécula
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article