Quantitation of TGF-ß proteins in mouse tissues shows reciprocal changes in TGF-ß1 and TGF-ß3 in normal vs neoplastic mammary epithelium.
Oncotarget
; 7(25): 38164-38179, 2016 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-27203217
ABSTRACT
Transforming growth factor-ßs (TGF-ßs) regulate tissue homeostasis, and their expression is perturbed in many diseases. The three isoforms (TGF-ß1, -ß2, and -ß3) have similar bioactivities in vitro but show distinct activities in vivo. Little quantitative information exists for expression of TGF-ß isoform proteins in physiology or disease. We developed an optimized method to quantitate protein levels of the three isoforms, using a Luminex® xMAP®-based multianalyte assay following acid-ethanol extraction of tissues. Analysis of multiple tissues and plasma from four strains of adult mice showed that TGF-ß1 is the predominant isoform with TGF-ß2 being ~10-fold lower. There were no sex-specific differences in isoform expression, but some tissues showed inter-strain variation, particularly for TGF-ß2. The only adult tissue expressing appreciable TGF-ß3 was the mammary gland, where its levels were comparable to TGF-ß1. In situ hybridization showed the luminal epithelium as the major source of all TGF-ß isoforms in the normal mammary gland. TGF-ß1 protein was 3-8-fold higher in three murine mammary tumor models than in normal mammary gland, while TGF-ß3 protein was 2-3-fold lower in tumors than normal tissue, suggesting reciprocal regulation of these isoforms in mammary tumorigenesis.
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MEDLINE
Assunto principal:
Fator de Crescimento Transformador beta
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Fator de Crescimento Transformador beta1
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Fator de Crescimento Transformador beta3
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Neoplasias Mamárias Experimentais
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article