Effects of alpha-tocopherol on gingival expression of inducible nitric oxide synthase in the rats with experimental periodontitis and diabetes.
Niger J Clin Pract
; 19(4): 480-5, 2016.
Article
em En
| MEDLINE
| ID: mdl-27251964
ABSTRACT
BACKGROUND:
The aim of this study was to investigate effects of α-tocopherol and/or insulin on the number of gingival inducible nitric oxide synthase (iNOS) positive cells in rats with experimental periodontitis with or without streptozotocin (STZ)-induced diabetes. MATERIALS ANDMETHODS:
A total of 60 Sprague-Dawley rats were divided into three groups Group I The group without diabetes; Group II The group with STZ-induced diabetes; Group III The group with STZ-induced diabetes receiving insulin therapy. All animals received anesthesia, and 3/0 silk suture was inserted around the mandibular molar teeth. All groups were divided into subgroups receiving saline (Groups IA, IIA, IIIA) and α-tocopherol injection (Groups IB, IIB, IIIB). After a period of 3 weeks, all rats were sacrificed, and the number of gingival iNOS positive cells was analyzed using image analysis software.RESULTS:
Applying α-tocopherol suppressed the number of gingival iNOS positive cells in Groups IB, IIB, and IIIB compared to application of saline (Groups IA, IIA, and IIIA) (P < 0.05). Numbers of gingival iNOS positive cells were found to be similar in the Groups I and III (P > 0.05).CONCLUSIONS:
Within limitations of the current study, this is the first study one may suggest that α-tocopherol may reduce oxidative damage in the gingiva of the rats with periodontitis with or without STZ-induced diabetes and increase effects of insulin.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Periodontite
/
Alfa-Tocoferol
/
Diabetes Mellitus Experimental
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Óxido Nítrico Sintase Tipo II
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Gengiva
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article