Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D.

Susanto, Olivia; Muinonen-Martin, Andrew J; Nobis, Max; Insall, Robert H

Susanto, Olivia; Muinonen-Martin, Andrew J; Nobis, Max; Insall, Robert H.

Afiliação

Susanto O; CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Muinonen-Martin AJ; Melanoma Clinic, Leeds Cancer Centre, St. James's University Hospital, Beckett Street, Leeds, LS9 7TF, UK.
Nobis M; CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Insall RH; CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK. r.insall@beatson.gla.ac.uk.

Methods Mol Biol ; 1407: 217-28, 2016.

Article em En | MEDLINE | ID: mdl-27271906

ABSTRACT

ABSTRACT

We describe three chemotaxis assays-Insall chambers, circular invasion assays, and 3D organotypic assays-that are particularly appropriate for measuring migration of cancer cells in response to gradients of soluble attractants. Each assay has defined advantages, and together they provide the best possible quantitative assessment of cancer chemotaxis.

Assuntos

Movimento Celular; Quimiotaxia; Neoplasias/metabolismo; Neoplasias/patologia; Animais; Técnicas de Cultura de Células; Linhagem Celular Transformada; Linhagem Celular Tumoral; Humanos; Ratos; Células Tumorais Cultivadas

Palavras-chave

Cell migration; Chemotaxis; Invasion; Melanoma; Metastasis; Organotypic

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Movimento Celular / Quimiotaxia / Neoplasias Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo

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PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Movimento Celular / Quimiotaxia / Neoplasias Idioma: En Ano de publicação: 2016 Tipo de documento: Article