Quantitative Monitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal Fluorescent Microscopy.

Xu, Xuehua; Yun, Michelle; Wen, Xi; Brzostowski, Joseph; Quan, Wei; Wang, Q Jane; Jin, Tian

Xu, Xuehua; Yun, Michelle; Wen, Xi; Brzostowski, Joseph; Quan, Wei; Wang, Q Jane; Jin, Tian.

Afiliação

Xu X; Chemotaxis Signaling Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, 5601 Fishers Lane, MSC 9806, Rockville, MD, USA. xxu@niaid.nih.gov.
Yun M; Chemotaxis Signaling Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, 5601 Fishers Lane, MSC 9806, Rockville, MD, USA.
Wen X; Chemotaxis Signaling Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, 5601 Fishers Lane, MSC 9806, Rockville, MD, USA.
Brzostowski J; Imaging Core Facility, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD, USA.
Quan W; Chemotaxis Signaling Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, 5601 Fishers Lane, MSC 9806, Rockville, MD, USA.
Wang QJ; Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Jin T; Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, 5601 Fishers Lane, MSC 9806, Rockville, MD, USA.

Methods Mol Biol ; 1407: 307-23, 2016.

Article em En | MEDLINE | ID: mdl-27271911

Assuntos

Microscopia Confocal; Imagem Molecular; Canais de Cátion TRPP/metabolismo; Proteínas ras/metabolismo; Linhagem Celular Tumoral; Fatores Quimiotáticos/metabolismo; Fatores Quimiotáticos/farmacologia; Ativação Enzimática; Transferência Ressonante de Energia de Fluorescência; Expressão Gênica; Genes Reporter; Células HL-60; Humanos; Microscopia Confocal/métodos; Fosforilação; Canais de Cátion TRPP/genética; Proteínas ras/genética

Palavras-chave

Confocal fluorescence microscopy; Förster resonance energy transfer (FRET); Phosphorylation of PKD1; Protein kinase D 1 (PKD1); Ras activation

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas ras / Microscopia Confocal / Canais de Cátion TRPP / Imagem Molecular Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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