A replicator-specific binding protein essential for site-specific initiation of DNA replication in mammalian cells.
Nat Commun
; 7: 11748, 2016 06 08.
Article
em En
| MEDLINE
| ID: mdl-27272143
ABSTRACT
Mammalian chromosome replication starts from distinct sites; however, the principles governing initiation site selection are unclear because proteins essential for DNA replication do not exhibit sequence-specific DNA binding. Here we identify a replication-initiation determinant (RepID) protein that binds a subset of replication-initiation sites. A large fraction of RepID-binding sites share a common G-rich motif and exhibit elevated replication initiation. RepID is required for initiation of DNA replication from RepID-bound replication origins, including the origin at the human beta-globin (HBB) locus. At HBB, RepID is involved in an interaction between the replication origin (Rep-P) and the locus control region. RepID-depleted murine embryonic fibroblasts exhibit abnormal replication fork progression and fewer replication-initiation events. These observations are consistent with a model, suggesting that RepID facilitates replication initiation at a distinct group of human replication origins.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Origem de Replicação
/
Proteínas de Ligação a DNA
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Replicação do DNA
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Mamíferos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article