Three New Escherichia coli Phages from the Human Gut Show Promising Potential for Phage Therapy.
PLoS One
; 11(6): e0156773, 2016.
Article
em En
| MEDLINE
| ID: mdl-27280590
ABSTRACT
With the emergence of multi-drug resistant bacteria the use of bacteriophages (phages) is gaining renewed interest as promising anti-microbial agents. The aim of this study was to isolate and characterize phages from human fecal samples. Three new coliphages, ɸAPCEc01, ɸAPCEc02 and ɸAPCEc03, were isolated. Their phenotypic and genomic characteristics, and lytic activity against biofilm, and in combination with ciprofloxacin, were investigated. All three phages reduced the growth of E. coli strain DPC6051 at multiplicity of infection (MOI) between 10-3 and 105. A cocktail of all three phages completely inhibited the growth of E. coli. The phage cocktail also reduced biofilm formation and prevented the emergence of phage-resistant mutants which occurred with single phage. When combined with ciprofloxacin, phage alone or in cocktail inhibited the growth of E. coli and prevented the emergence of resistant mutants. These three new phages are promising biocontrol agents for E. coli infections.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Colífagos
/
Trato Gastrointestinal
/
Escherichia coli
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Infecções por Escherichia coli
/
Fezes
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Terapia por Fagos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article