Piperidine-based glycodendrons as protein N-glycan prosthetics.
Bioorg Med Chem
; 24(20): 4791-4800, 2016 10 15.
Article
em En
| MEDLINE
| ID: mdl-27283789
ABSTRACT
The generation of homogeneously glycosylated proteins is essential for defining glycoform-specific activity and improving protein-based therapeutics. We present a novel glycodendron prosthetic which can be site-selectively appended to recombinant proteins to create 'N-glycosylated' glycoprotein mimics. Using computational modeling, we designed the dendrimer scaffold and protein attachment point to resemble the native N-glycan architecture. Three piperidine-melamine glycodendrimers were synthesized via a chemoenzymatic route and attached to human growth hormone and the Fc region of human IgG. These products represent a new class of engineered biosimilars bearing novel glycodendrimer structures.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Piperidinas
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Polissacarídeos
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Proteínas
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Dendrímeros
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article