Crystal structures of two monomeric triosephosphate isomerase variants identified via a directed-evolution protocol selecting for L-arabinose isomerase activity.

Krause, Mirja; Kiema, Tiila Riikka; Neubauer, Peter; Wierenga, Rik K

Krause, Mirja; Kiema, Tiila Riikka; Neubauer, Peter; Wierenga, Rik K.

Afiliação

Krause M; Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, Ackerstrasse 76, ACK 24, Berlin, Germany.
Kiema TR; Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu, FIN-90014 Oulu, Finland.
Neubauer P; Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, Ackerstrasse 76, ACK 24, Berlin, Germany.
Wierenga RK; Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu, FIN-90014 Oulu, Finland.

Acta Crystallogr F Struct Biol Commun ; 72(Pt 6): 490-9, 2016 06.

Article em En | MEDLINE | ID: mdl-27303904

Assuntos

Aldose-Cetose Isomerases/química; Triose-Fosfato Isomerase/química; Aldose-Cetose Isomerases/genética; Dicroísmo Circular; Cristalização; Cristalografia por Raios X; Evolução Molecular Direcionada; Conformação Proteica; Triose-Fosfato Isomerase/genética

Palavras-chave

TIM barrel; enzyme engineering; non-natural enzymes; structure-based rational design; triosephosphate isomerase

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Triose-Fosfato Isomerase / Aldose-Cetose Isomerases Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Triose-Fosfato Isomerase / Aldose-Cetose Isomerases Idioma: En Ano de publicação: 2016 Tipo de documento: Article