Thermal and clinical performance of a closed device designed for human oocyte vitrification based on the optimization of the warming rate.
Cryobiology
; 73(1): 40-6, 2016 08.
Article
em En
| MEDLINE
| ID: mdl-27312427
ABSTRACT
Although it was qualitatively pointed out by Fahy et al. (1984), the key role of the warming rates in non-equillibrium vitrification has only recently been quantitatively established for murine oocytes by Mazur and Seki (2011). In this work we study the performance of a closed vitrification device designed under the new paradigm, for the vitrification of human oocytes. The vitrification carrier consists of a main straw in which a specifically designed capillary is mounted and where the oocytes are loaded by aspiration. It can be hermetically sealed before immersion in liquid nitrogen for vitrification, and it is warmed in a sterile water bath at 37 °C. Measured warming rates achieved with this design were of 600.000 ºC/min for a standard DMEM solution and 200.000 ºC/min with the vitrification solution for human oocytes. A cohort of 143 donor MII sibling human oocytes was split into two groups control (fresh) and vitrified with SafeSpeed device. Similar results were found in both groups survival (97.1%), fertilization after ICSI (74.7% in control vs. 77.3% in vitrified) and good quality embryos at day three (54.3% in control vs. 58.1% in vitrified) were settled as performance indicators. The pregnancy rate was 3/6 (50%) for the control, 2/3 (66%) for vitrified and 4/5 (80%) for mixed transfers.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fertilização in vitro
/
Criopreservação
/
Vitrificação
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article