Developments in Plant Negative-Strand RNA Virus Reverse Genetics.
Annu Rev Phytopathol
; 54: 469-98, 2016 08 04.
Article
em En
| MEDLINE
| ID: mdl-27359368
ABSTRACT
Twenty years ago, breakthroughs for reverse genetics analyses of negative-strand RNA (NSR) viruses were achieved by devising conditions for generation of infectious viruses in susceptible cells. Recombinant strategies have subsequently been engineered for members of all vertebrate NSR virus families, and research arising from these advances has profoundly increased understanding of infection cycles, pathogenesis, and complexities of host interactions of animal NSR viruses. These strategies also permitted development of many applications, including attenuated vaccines and delivery vehicles for therapeutic and biotechnology proteins. However, for a variety of reasons, it was difficult to devise procedures for reverse genetics analyses of plant NSR viruses. In this review, we discuss advances that have circumvented these problems and resulted in construction of a recombinant system for Sonchus yellow net nucleorhabdovirus. We also discuss possible extensions to other plant NSR viruses as well as the applications that may emanate from recombinant analyses of these pathogens.
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1
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
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Rhabdoviridae
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Genética Reversa
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article