Sequence-Defined Oligomers from Hydroxyproline Building Blocks for Parallel Synthesis Applications.
Angew Chem Int Ed Engl
; 55(33): 9529-33, 2016 08 08.
Article
em En
| MEDLINE
| ID: mdl-27365192
ABSTRACT
The functionality of natural biopolymers has inspired significant effort to develop sequence-defined synthetic polymers for applications including molecular recognition, self-assembly, and catalysis. Conjugation of synthetic materials to biomacromolecules has played an increasingly important role in drug delivery and biomaterials. We developed a controlled synthesis of novel oligomers from hydroxyproline-based building blocks and conjugated these materials to siRNA. Hydroxyproline-based monomers enable the incorporation of broad structural diversity into defined polymer chains. Using a perfluorocarbon purification handle, we were able to purify diverse oligomers through a single solid-phase extraction method. The efficiency of synthesis was demonstrated by building 14 unique trimers and 4 hexamers from 6 diverse building blocks. We then adapted this method to the parallel synthesis of hundreds of materials in 96-well plates. This strategy provides a platform for the screening of libraries of modified biomolecules.
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MEDLINE
Assunto principal:
Poliuretanos
/
Hidroxiprolina
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article