Analysis of single nucleotide polymorphism among Varicella-Zoster Virus and identification of vaccine-specific sites.
Virology
; 496: 277-286, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27376245
ABSTRACT
Varicella-zoster virus (VZV) is a causative agent for chickenpox and zoster. Live attenuated vaccines have been developed based on Oka and MAV/06 strains. In order to understand the molecular mechanisms of attenuation, complete genome sequences of vaccine and wild-type strains were compared and single nucleotide polymorphism (SNP) was analyzed. ORF22 and ORF62 contained the highest number of SNPs. The detailed analysis of the SNPs suggested 24 potential vaccine-specific sites. All the mutational events found in vaccine-specific sites were transitional, and most of them were substitution of AT to GC pair. Interestingly, 18 of the vaccine-specific sites of the vaccine strains appeared to be genetically heterogeneous. The probability of a single genome of vaccine strain to contain all 24 vaccine-type sequences was calculated to be less than 4%. The average codon adaptation index (CAI) value of the vaccine strains was significantly lower than the CAI value of the clinical strains.
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Base de dados:
MEDLINE
Assunto principal:
Genoma Viral
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Herpesvirus Humano 3
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Vacina contra Varicela
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Polimorfismo de Nucleotídeo Único
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Epitopos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article